sigM [2017-8-11 16:0:3]

RNA polymerase ECF-type sigma factor SigM, required for adaptation to inhibitors of peptidoglycan synthesis

Locus: BSU09520

Isoelectric point: 6.77

Molecular weight: 19.26 kDa

Protein length: 163 aa

Gene length: 489 bp

Function: adaptation to inhibitors of peptidoglycan synthesis

Product: RNA polymerase ECF-type sigma factor SigM

Essential: no

Synonyms: yhdM

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → Transcription → Sigma factors
• Information processing → Regulation of gene expression → Sigma factors and their control → Sigma factors
• Lifestyles → Coping with stress → Resistance against toxins/ antibiotics
• Lifestyles → Coping with stress → Cell envelope stress proteins (controlled by SigM, V, W, X, Y)

Gene

Coordinates: 1,029,577 → 1,030,068

Phenotypes of a mutant

• SigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
• sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
• increased sensitivity towards beta-lactam antibiotics PubMed
• a sigM murJ double mutant is not viable (due to the lack of both lipid II flippases) PubMed

The protein

Protein family

• sigma-70 factor family, ECF subfamily (according to Swiss-Prot)

Effectors of protein activity

• expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
• shortage of available bactoprenol causes SigM activation PubMed

Structure

• 5WUQ (SigW in complex with the cytoplasmic domain of RsiW, 32% identity) PubMed

Additional information

• Expression of the SigM regulon in increased in ugtP mutants PubMed

Expression and Regulation

Operons

• Genes: sigM-yhdL-yhdK
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigM: sigma factor, PubMed, in SigM regulon

  Regulation

  • induced by glucose PubMed

  Additional information

  • expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed

Biological materials

Mutant

• 1A906 (sigM::kan), PubMed, available at BGSC
• BP129 (sigM::aphA3), available in Fabian Commichau's lab

Labs working on this gene/protein

• John Helmann, Cornell University, USA Homepage

References

Reviews

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The SigM regulon

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Other original publications

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